DNA replication (WP983)

Bos taurus

Studies in the past decade have suggested that the basic mechanism of DNA replication initiation is conserved in all kingdoms of life. Initiation in unicellular eukaryotes, in particular Saccharomyces cerevisiae (budding yeast), is well understood, and has served as a model for studies of DNA replication initiation in multicellular eukaryotes, including humans. In general terms, the first step of initiation is the binding of the replication initiator to the origin of replication. The replicative helicase is then assembled onto the origin, usually by a helicase assembly factor. Either shortly before or shortly after helicase assembly, some local unwinding of the origin of replication occurs in a region rich in adenine and thymine bases (often termed a DNA unwinding element, DUE). The unwound region provides the substrate for primer synthesis and initiation of DNA replication. The best-defined eukaryotic origins are those of S. cerevisiae, which have well-conserved sequence elements for initiator binding, DNA unwinding and binding of accessory proteins. In multicellular eukaryotes, unlike S. cerevisiae, these loci appear not to be defined by the presence of a DNA sequence motif. Indeed, choice of replication origins in a multicellular eukaryote may vary with developmental stage and tissue type. In cell-free models of metazoan DNA replication, such as the one provided by Xenopus egg extracts, there are only limited DNA sequence specificity requirements for replication initiation. Source description: http://www.reactome.org/content/detail/69306.

Authors

Thomas Kelder , Christine Chichester , Martina Summer-Kutmon , Egon Willighagen , Eric Weitz , and Kristina Hanspers

Activity

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Organisms

Bos taurus

Communities

Annotations

Pathway Ontology

DNA replication pathway

Participants

Label Type Compact URI Comment
ADP Metabolite cas:58-64-0
UTP Metabolite chebi:15713
dGTP Metabolite cas:2564-35-4
CTP Metabolite cas:65-47-4
ATP Metabolite cas:1927-31-7
dATP Metabolite cas:1927-31-7
GTP Metabolite cas:2564-35-4
dCTP Metabolite cas:2056-98-6
dUTP Metabolite chebi:17625
ORC5L GeneProduct ensembl:ENSBTAG00000040058 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:5001
RFC1 GeneProduct ensembl:ENSBTAG00000014515 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:5981
POLD2 GeneProduct ensembl:ENSBTAG00000012241 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:5425
RFC4 GeneProduct ensembl:ENSBTAG00000014727 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:5984
RPA1 GeneProduct ensembl:ENSBTAG00000009711 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:6117
PCNA GeneProduct ensembl:ENSBTAG00000006065 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:5111
ORC6 GeneProduct ensembl:ENSBTAG00000001872 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:23594
MCM5 GeneProduct ensembl:ENSBTAG00000015595 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:4174
CDC45L GeneProduct ensembl:ENSBTAG00000004286 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:8318
CDC7L1 GeneProduct ensembl:ENSBTAG00000016307 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:8317
GMNN GeneProduct ensembl:ENSBTAG00000017329 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:51053
POLD4 GeneProduct ensembl:ENSBTAG00000017951 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:57804
MCM6 GeneProduct ensembl:ENSBTAG00000015172 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:4175
CDT1 GeneProduct ensembl:ENSBTAG00000000638 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:81620
RFC5 GeneProduct ensembl:ENSBTAG00000007137 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:5985
DBF4 GeneProduct ensembl:ENSBTAG00000014711 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:10926
RPA2 GeneProduct ensembl:ENSBTAG00000006225 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:6118
POLD1 GeneProduct ensembl:ENSBTAG00000011083 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:5424
MCM2 GeneProduct ensembl:ENSBTAG00000014380 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:4171
CDK2 GeneProduct ensembl:ENSBTAG00000004021 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:1017
RPA3 GeneProduct ensembl:ENSBTAG00000013168 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:6119
PRIM1 GeneProduct ensembl:ENSBTAG00000044000 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:5557
PRIM2 GeneProduct ensembl:ENSBTAG00000007937 HomologyConvert: Homo sapiens to Bos taurus: Original ID = En:ENSG00000146143
ORC2 GeneProduct ensembl:ENSBTAG00000031473 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:4999
RFC3 GeneProduct ensembl:ENSBTAG00000010787 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:5983
MCM10 GeneProduct ensembl:ENSBTAG00000016406 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:55388
POLD3 GeneProduct ensembl:ENSBTAG00000016869 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:10714
POLA2 GeneProduct ensembl:ENSBTAG00000011455 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:23649
ORC4 GeneProduct ensembl:ENSBTAG00000015291 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:5000
MCM7 GeneProduct ensembl:ENSBTAG00000030965 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:4176
MCM4 GeneProduct ensembl:ENSBTAG00000017021 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:4173
POLE2 GeneProduct ensembl:ENSBTAG00000004931 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:5427
UBA52 GeneProduct ensembl:ENSBTAG00000007737 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:7311
CDC6 GeneProduct ensembl:ENSBTAG00000010384 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:990
ORC3 GeneProduct ensembl:ENSBTAG00000016969 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:23595
POLE GeneProduct ensembl:ENSBTAG00000000590 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:5426
ORC1 GeneProduct ensembl:ENSBTAG00000002719 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:4998
RFC2 GeneProduct ensembl:ENSBTAG00000018589 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:5982
MCM3 GeneProduct ensembl:ENSBTAG00000010721 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:4172
POLA1 GeneProduct ensembl:ENSBTAG00000008170 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:5422

References