De novo biosynthesis of purine nucleotides (WP203)
Saccharomyces cerevisiae
Purine nucleotides participate in many aspects of cellular metabolism including the structure of DNA and RNA, serving as enzyme cofactors, functioning in cellular signaling, acting as phosphate group donors, and generating cellular energy. Maintenance of the proper balance of intracellular pools of these nucleotides is critical to normal function. This occurs through a combination of de novo biosynthesis and salvage pathways for pre-existing purine bases, nucleosides and nucleotides. The de novo biosynthetic pathway for purine nucleotides is highly conserved among organisms, but its regulation and the organization of the genes encoding the enzymes vary. This fourteen step pathway contains ten steps that branch at IMP to form AMP and GMP, each in two steps. Regulation of the pathway has been well studied in microbes such as Escherichia coli, Bacillus subtilis and Saccharomyces cerevisiae, but little is known about its regulation in higher eukaryotes (metazoa, and plants). Source: https://pathway.yeastgenome.org/
Authors
Meredith Braymer , Christine Chichester , Egon Willighagen , Martina Summer-Kutmon , Eric Weitz , and Kristina HanspersActivity
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Organisms
Saccharomyces cerevisiaeCommunities
Annotations
Pathway Ontology
nucleotide metabolic pathway de novo purine biosynthetic pathway| Label | Type | Compact URI | Comment |
|---|---|---|---|
| IMP | Metabolite | chebi:58053 | |
| ADP | Metabolite | cas:58-64-0 | |
| 5-phospho-β-D-ribosyl-glycineamide | Metabolite | chebi:58457 | |
| L-glutamate | Metabolite | cas:56-86-0 | |
| dGDP | Metabolite | pubchem.compound:644 | |
| ATP | Metabolite | cas:1927-31-7 | |
| NADH | Metabolite | cas:53-84-9 | |
| 5-phosphoribosyl-N-formylglycineamidine | Metabolite | chebi:18413 | |
| L-glutamine | Metabolite | cas:56-85-9 | |
| GDP | Metabolite | cas:146-91-8 | |
| L-glycine | Metabolite | cas:56-40-6 | |
| H+ | Metabolite | chebi:15378 | |
| phosphate | Metabolite | cas:14265-44-2 | |
| diphosphate | Metabolite | cas:2466-09-3 | |
| GTP | Metabolite | cas:2564-35-4 | |
| dGTP | Metabolite | chebi:61429 | |
| AICAR | Metabolite | cas:3031-94-5 | |
| xanthosine-5-phosphate | Metabolite | cas:523-98-8 | |
| formate | Metabolite | chebi:15740 | |
| 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | Metabolite | chebi:58467 | |
| GMP | Metabolite | cas:85-32-5 | |
| 10-formyl-THF | Metabolite | cas:2800-34-2 | |
| 5-phospho-α-D-ribose 1-diphosphate | Metabolite | chebi:58017 | |
| H2O | Metabolite | chebi:15377 | |
| 5-phospho-β-D-ribosylamine | Metabolite | chebi:58681 | |
| CO2 | Metabolite | chebi:16526 | |
| 5-aminoimidazole ribonucleotide | Metabolite | chebi:137981 | |
| 1-(5-phosphoribosyl)-4-carboxy-5-aminoimidazole | Metabolite | chebi:58564 | |
| L-aspartate | Metabolite | chebi:29991 | |
| AMP | Metabolite | chebi:456215 | |
| 5'-P-ribosyl-4-(N-succinocarboxamide)-5-aminoimidazole | Metabolite | chebi:58443 | |
| fumarate | Metabolite | chebi:29806 | |
| NAD+ | Metabolite | chebi:57540 | |
| dADP | Metabolite | chebi:57667 | |
| adenylo-succinate | Metabolite | chebi:57567 | |
| dATP | Metabolite | chebi:61404 | |
| ADE13 | GeneProduct | sgd:S000004351 | |
| YNK1 | GeneProduct | sgd:S000001550 | |
| ADE16 | GeneProduct | sgd:S000004018 | |
| ADE8 | GeneProduct | sgd:S000002816 | |
| GUK1 | GeneProduct | sgd:S000001550 | |
| ADK2 | GeneProduct | sgd:S000000972 | |
| ADK1 | GeneProduct | sgd:S000002634 | |
| RNR3 | GeneProduct | sgd:S000001328 | |
| ADE5,7 | GeneProduct | sgd:S000003203 | |
| ADE6 | GeneProduct | sgd:S000003293 | |
| ADE2 | GeneProduct | sgd:S000005654 | |
| RNR1 | GeneProduct | sgd:S000000872 | |
| ADE12 | GeneProduct | sgd:S000005164 | |
| ADE1 | GeneProduct | sgd:S000000070 | |
| RNR2 | GeneProduct | sgd:S000003563 | |
| IMD4 | GeneProduct | sgd:S000004520 | |
| GUA1 | GeneProduct | sgd:S000004830 | |
| IMD2 | GeneProduct | sgd:S000001259 | |
| IMD3 | GeneProduct | sgd:S000004424 | |
| RNR4 | GeneProduct | sgd:S000003412 | |
| ADE17 | GeneProduct | sgd:S000004727 | |
| ADE4 | GeneProduct | sgd:S000004915 |
References
- Transcriptional regulation of the yeast gmp synthesis pathway by its end products. Escobar-Henriques M, Daignan-Fornier B. J Biol Chem. 2001 Jan 12;276(2):1523–30. PubMed Europe PMC Scholia
- Yeast AMP pathway genes respond to adenine through regulated synthesis of a metabolic intermediate. Rébora K, Desmoucelles C, Borne F, Pinson B, Daignan-Fornier B. Mol Cell Biol. 2001 Dec;21(23):7901–12. PubMed Europe PMC Scholia
- Revisiting purine-histidine cross-pathway regulation in Saccharomyces cerevisiae: a central role for a small molecule. Rébora K, Laloo B, Daignan-Fornier B. Genetics. 2005 May;170(1):61–70. PubMed Europe PMC Scholia
- URL: https://pathway.yeastgenome.org/YEAST/NEW-IMAGE?type=PATHWAY&object=DENOVOPURINE2-PWY