mRNA Processing (WP1142)

Canis familiaris

This process describes the conversion of precursor messenger RNA into mature messenger RNA (mRNA). The pre-mRNA molecule undergoes three main modifications. These modifications are 5' capping, 3' polyadenylation, and RNA splicing, which occur in the cell nucleus before the RNA is translated. 5' Capping: Capping of the pre-mRNA involves the addition of 7-methylguanosine (m7G) to the 5' end. The cap protects the 5' end of the primary RNA transcript from attack by ribonucleases that have specificity to the 3'5' phosphodiester bonds. 3' Processing: The pre-mRNA processing at the 3' end of the RNA molecule involves cleavage of its 3' end and then the addition of about 200 adenine residues to form a poly(A) tail. As the poly(A) tails is synthesised, it binds multiple copies of poly(A) binding protein, which protects the 3'end from ribonuclease digestion. Splicing: RNA splicing is the process by which introns, regions of RNA that do not code for protein, are removed from the pre-mRNA and the remaining exons connected to re-form a single continuous molecule. Description adapted from Wikipedia: http://en.wikipedia.org/wiki/Post-transcriptional_modification Pathway adapted from http://www.reactome.org.

Authors

Kristina Hanspers , Daniela Digles , Martina Summer-Kutmon , and Alex Pico

Activity

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Organisms

Canis familiaris

Communities

Annotations

Pathway Ontology

spliceosome pathway transcription pathway

Participants

Label Type Compact URI Comment
GMP Metabolite cas:85-32-5
ATP Metabolite cas:1927-31-7
PRPF4 GeneProduct ensembl:ENSCAFG00000003224 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:9128
SNRPA1 GeneProduct ensembl:ENSCAFG00000010564 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6627
RBM17 GeneProduct ensembl:ENSCAFG00000005206 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:84991
SNRPD3 GeneProduct ensembl:ENSCAFG00000013864 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6634
NCBP2 GeneProduct ensembl:ENSCAFG00000013100 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:22916
RNGTT GeneProduct ensembl:ENSCAFG00000003089 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:8732
SNRPD1 GeneProduct ensembl:ENSCAFG00000018247 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6632
SF3A2 GeneProduct ensembl:ENSCAFG00000019414 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:8175
SNRPD2 GeneProduct ensembl:ENSCAFG00000004403 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6633
XRN2 GeneProduct ensembl:ENSCAFG00000005185 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:22803
DHX16 GeneProduct ensembl:ENSCAFG00000000427 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:8449
HNRNPM GeneProduct ensembl:ENSCAFG00000018524 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:4670
HNRPA1 GeneProduct ensembl:ENSCAFG00000017316 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:3178
CELF2 GeneProduct ensembl:ENSCAFG00000004973 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10659
SPOP GeneProduct ensembl:ENSCAFG00000016942 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:8405
HNRNPA2B1 GeneProduct ensembl:ENSCAFG00000002885 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:3181
PHF5A GeneProduct ensembl:ENSCAFG00000001090 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:84844
SF3B5 GeneProduct ensembl:ENSCAFG00000032495 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:83443
HNRNPR GeneProduct ensembl:ENSCAFG00000013308 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10236
PRPF6 GeneProduct ensembl:ENSCAFG00000013165 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:24148
NSEP1 GeneProduct ensembl:ENSCAFG00000002541 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:4904
SRSF9 GeneProduct ensembl:ENSCAFG00000010314 PMID: 12738786, PMID: 12024014 (associated with hnRNP A1 splicing)
Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:8683
SUGP1 GeneProduct ensembl:ENSCAFG00000014314 inferred from: SURP 2, G patch protein, UniProt:O60378
Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:57794
SSFA1 GeneProduct ensembl:ENSCAFG00000018660 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:4809
SF3B3 GeneProduct ensembl:ENSCAFG00000020151 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:23450
PRPF40A GeneProduct ensembl:ENSCAFG00000005827 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:55660
PSKH1 GeneProduct ensembl:ENSCAFG00000020335 PMID: 12466556
Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:5681
SNRPB2 GeneProduct ensembl:ENSCAFG00000024887 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6629
HNRNPH2 GeneProduct ensembl:ENSCAFG00000017693 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:3188
SNRNP40 GeneProduct ensembl:ENSCAFG00000011149 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:9410
HNRNPU GeneProduct ensembl:ENSCAFG00000015835 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:3192
LSM7 GeneProduct ensembl:ENSCAFG00000019409 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:51690
DDX1 GeneProduct ensembl:ENSCAFG00000003686 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:1653
SRPK1 GeneProduct ensembl:ENSCAFG00000001364 PMID: 12773558
Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6732
U2AF1 GeneProduct ensembl:ENSCAFG00000010572 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:7307
TRA2B GeneProduct ensembl:ENSCAFG00000013345 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6434
SRPK2 GeneProduct ensembl:ENSCAFG00000004057 PMID: 12773558
Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6733
SUPT5H GeneProduct ensembl:ENSCAFG00000005469 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6829
SRP54 GeneProduct ensembl:ENSCAFG00000013331 PMID: 14729963
Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6729
PRPF4B GeneProduct ensembl:ENSCAFG00000009381 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:8899
CELF1 GeneProduct ensembl:ENSCAFG00000008452 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10658
SRSF1 GeneProduct ensembl:ENSCAFG00000017430 Antagonist splicing factor
Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6426
CLP1 GeneProduct ensembl:ENSCAFG00000007815 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10978
SNRPF GeneProduct ensembl:ENSCAFG00000031151 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6636
METTL3 GeneProduct ensembl:ENSCAFG00000005657 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:56339
SRSF6 GeneProduct ensembl:ENSCAFG00000009289 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6431
NXF1 GeneProduct ensembl:ENSCAFG00000015594 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10482
SF3B2 GeneProduct ensembl:ENSCAFG00000013033 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10992
SFPQ GeneProduct ensembl:ENSCAFG00000017608 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6421
RBM5 GeneProduct ensembl:ENSCAFG00000010866 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10181
PRPF3 GeneProduct ensembl:ENSCAFG00000011820 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:9129
CSTF3 GeneProduct ensembl:ENSCAFG00000007264 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:1479
PAPOLA GeneProduct ensembl:ENSCAFG00000017783 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10914
HNRNPD GeneProduct ensembl:ENSCAFG00000025045 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:3184
CPSF3 GeneProduct ensembl:ENSCAFG00000003375 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:51692
HNRNPAB GeneProduct ensembl:ENSCAFG00000000241 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:3182
CLASRP GeneProduct ensembl:ENSCAFG00000004587 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:11129
DICER1 GeneProduct ensembl:ENSCAFG00000017701 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:23405
PABPN1 GeneProduct ensembl:ENSCAFG00000011482 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:8106
PRMT1 GeneProduct ensembl:ENSCAFG00000003589 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:3276
CPSF4 GeneProduct ensembl:ENSCAFG00000015036 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10898
SFSWAP GeneProduct ensembl:ENSCAFG00000006625 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6433
SRSF3 GeneProduct ensembl:ENSCAFG00000001414 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6428
SF3A1 GeneProduct ensembl:ENSCAFG00000012559 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10291
CD2BP2 GeneProduct ensembl:ENSCAFG00000016943 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10421
TMED10 GeneProduct ensembl:ENSCAFG00000017034 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10972
CPSF2 GeneProduct ensembl:ENSCAFG00000010909 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:53981
CPSF1 GeneProduct ensembl:ENSCAFG00000001600 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:29894
SRSF2 GeneProduct ensembl:ENSCAFG00000005147 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6427
PPM1G GeneProduct ensembl:ENSCAFG00000004923 PMID: 12773558
Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:5496
NUDT21 GeneProduct ensembl:ENSCAFG00000002667 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:11051
SF3B4 GeneProduct ensembl:ENSCAFG00000011617 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10262
CSTF2 GeneProduct ensembl:ENSCAFG00000017551 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:1478
DHX38 GeneProduct ensembl:ENSCAFG00000020228 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:9785
HNRPC GeneProduct ensembl:ENSCAFG00000018380 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:3183
SREK1 GeneProduct ensembl:ENSCAFG00000007560 PMID: 14559993. SRrp86 is a unique member of the SR protein superfamily containing one RNA recognition motif and two serine-arginine (SR)-rich domains separated by an unusual glutamic acid-lysine (EK)-rich region. Previously, we showed that SRrp86 could regulate alternative splicing by both positively and negatively modulating the activity of other SR proteins and that the unique EK domain could inhibit both constitutive and alternative splicing. These functions were most consistent with the model in which SRrp86 functions by interacting with and thereby modulating the activity of target proteins. To identify the specific proteins that interact with SRrp86, we used a yeast two-hybrid library screen and immunoprecipitation coupled to mass spectrometry. We show that SRrp86 interacts with all of the core SR proteins, as well as a subset of other splicing regulatory proteins, including SAF-B, hnRNP G, YB-1, and p72. In contrast to previous results that showed activation of SRp20 by SRrp86, we now show that SAF-B, hnRNP G, and 9G8 all antagonize the activity of SRrp86. Overall, we conclude that not only does SRrp86 regulate SR protein activity but that it is, in turn, regulated by other splicing factors to control alternative splice site selection.
Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:140890
SRSF4 GeneProduct ensembl:ENSCAFG00000011669 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6429
CLK2 GeneProduct ensembl:ENSCAFG00000016969 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:1196
PRPF8 GeneProduct ensembl:ENSCAFG00000019221 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10594
SF3A3 GeneProduct ensembl:ENSCAFG00000003222 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10946
SNRPN GeneProduct ensembl:ENSCAFG00000030577 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6638
CLK4 GeneProduct ensembl:ENSCAFG00000000248 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:57396
SF3B1 GeneProduct ensembl:ENSCAFG00000010826 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:23451
PRMT2 GeneProduct ensembl:ENSCAFG00000012237 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:3275
DDX20 GeneProduct ensembl:ENSCAFG00000013674 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:11218
NONO GeneProduct ensembl:ENSCAFG00000017071 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:4841
SRSF5 GeneProduct ensembl:ENSCAFG00000016589 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6430
PRPF18 GeneProduct ensembl:ENSCAFG00000004735 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:8559
CSTF2T GeneProduct ensembl:ENSCAFG00000015567 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:23283
PTBP1 GeneProduct ensembl:ENSCAFG00000019693 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:5725
CSTF1 GeneProduct ensembl:ENSCAFG00000011914 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:1477
HNRPL GeneProduct ensembl:ENSCAFG00000005652 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:3191
U2AF2 GeneProduct ensembl:ENSCAFG00000002551 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:11338
SRSF7 GeneProduct ensembl:ENSCAFG00000029620 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6432
DHX9 GeneProduct ensembl:ENSCAFG00000013205 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:1660
SUGP2 GeneProduct ensembl:ENSCAFG00000014431 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10147
SRRM1 GeneProduct ensembl:ENSCAFG00000012933 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10250
RNMT GeneProduct ensembl:ENSCAFG00000000172 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:8731
SNRPE GeneProduct ensembl:ENSCAFG00000002085 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6635
CDC40 GeneProduct ensembl:ENSCAFG00000003868 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:51362
RBMX GeneProduct ensembl:ENSCAFG00000018962 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:27316
SNRPB GeneProduct ensembl:ENSCAFG00000031109 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6628
SMC1A GeneProduct ensembl:ENSCAFG00000016241 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:8243
SNRNP70 GeneProduct ensembl:ENSCAFG00000003711 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6625
TXNL4A GeneProduct ensembl:ENSCAFG00000000008 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10907
NCBP1 GeneProduct ensembl:ENSCAFG00000002445 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:4686
DHX8 GeneProduct ensembl:ENSCAFG00000014556 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:1659
CLK1 GeneProduct ensembl:ENSCAFG00000011929 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:1195
CLK3 GeneProduct ensembl:ENSCAFG00000017904 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:1198
PTBP2 GeneProduct ensembl:ENSCAFG00000020080 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:58155
EFTUD2 GeneProduct ensembl:ENSCAFG00000014065 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:9343
RBM39 GeneProduct ensembl:ENSCAFG00000008327 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:9584
SNRPA GeneProduct ensembl:ENSCAFG00000005091 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:6626
HNRNPH1 GeneProduct ensembl:ENSCAFG00000000336 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:3187
FUS GeneProduct ensembl:ENSCAFG00000016862 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:2521
RNPS1 GeneProduct ensembl:ENSCAFG00000019397 PMID: 14729963
Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:10921
DHX15 GeneProduct ensembl:ENSCAFG00000016505 Homology Mapping from Homo sapiens to Canis familiaris: Original ID = L:1665

References

  1. mRNA nuclear export at a glance. Carmody SR, Wente SR. J Cell Sci. 2009 Jun 15;122(Pt 12):1933–7. PubMed Europe PMC Scholia